To prepare for a resting metabolic rate (RMR) test, fast for at least 6 to 8 hours (water is fine), skip exercise that morning and avoid strenuous workouts for 24 hours, and stay away from caffeine, nicotine and alcohol beforehand. Book a morning slot, sleep well, and arrive rested. Protocols differ a little from one lab to the next, so the written instructions from your testing site always win. The rest of this guide explains the numbers behind each rule, so you can follow them sensibly and know what to do if something goes wrong.
TL;DR
- Fast at least 6 hours, and 8 if your site says so. Drink water, and skip calories and caffeine.
- No strenuous exercise for 24 hours, and none at all the morning of the test. Avoid alcohol for 24 hours.
- Skip caffeine, nicotine and stimulant supplements. Windows run from 3 to 24 hours depending on the protocol.
- Keep taking prescribed medications unless your clinician says otherwise, and tell the tester what you take.
- If you ate, had coffee or trained hard, tell the tester before you start. A rescheduled test beats a misleading number.
Table of Contents
- Why preparation changes the result
- Fasting: how long, and what you can drink
- Exercise: what counts and for how long
- Caffeine, nicotine, alcohol and stimulants
- Medications and supplements
- The day before: sleep, stress and timing
- What happens at the appointment
- What to do if you ate, trained or had caffeine
- Pairing an RMR test with a DEXA scan
- Our perspective
- Book an RMR or DEXA appointment near you
- FAQ
- Sources
Why preparation changes the result
An RMR test uses indirect calorimetry: you breathe into a mask or under a canopy, and the device estimates calories burned at rest from your oxygen use and carbon dioxide output. The test is only meaningful if your body is at a true resting baseline. Food, stimulants and physical activity all acutely change metabolic rate, which is why a systematic review for the Journal of the American Dietetic Association stresses standardized preparation for indirect calorimetry.
The practical stakes: you will likely use the number to set a calorie target. If a latte or a hard leg day inflated it, your target is too high, and a plan built on it will quietly stall. If you want the bigger picture of how the measurement fits into weight management, see our guide on RMR versus BMR and when to measure.
Fasting: how long, and what you can drink
This is the rule with the most variation, because each organization sets its own window:
| Source | Fasting guidance |
|---|---|
| UC Davis Sports Medicine | No food, caloric beverages or caffeine for at least 6 hours; water allowed |
| Ohio State Exercise Science Laboratory | Fast at least 8 hours; try to drink 1 to 2 cups of water before arriving |
| Academy of Nutrition and Dietetics (2014) | At least 7 hours for healthy adults, to minimize the thermic effect of feeding |
| ACSM Current Sports Medicine Reports (2008) | At least 6 hours when a strict resting state is needed |
The common thread is that digesting a meal raises your energy use, and the test is trying to capture what your body burns when it is not digesting. Six hours is the floor in these sources. If your clinic says 8 or 12, follow it.
A simple plan: eat an ordinary dinner, finish eating a few hours before bed, and book an early appointment so the fast is mostly sleep. Water is allowed by these protocols, and drinking a little beforehand helps you arrive comfortable. Clinic-facing guidance on fasting requirements for lab testing explains why the 8 to 12 hour range is standard across fasting-dependent tests, which is useful context if you are also getting bloodwork the same morning.
Do not stretch the fast far past what your site asks. A research protocol notes that readings can shift if someone fasted much longer than 15 hours, and you are more likely to feel lightheaded when you stand up afterward.
Exercise: what counts and for how long
Hard training raises metabolism well after you stop, so the rule is stricter than "no workout right before."
- Ohio State: avoid strenuous exercise for more than 24 hours before the test (source).
- UC Davis: no exercise the morning of the test, and avoid intense stress the day before (source).
- ACSM (2008): two hours of rest is enough after moderate aerobic activity, but vigorous resistance training should be avoided for 14 hours before measurement (source).
If you lift heavy or run hard, the safe move is to take the day before off, or keep it to an easy walk. Move your session to after the test, not before. A gentle walk to the clinic is fine, but arrive early enough that you are not rushing. A hurried commute raises your heart rate and breathing, and most sites will have you rest quietly before the measurement starts anyway.
If you are also planning a VO2 max test, the rest rules overlap but are not identical. Our VO2 max test preparation guide covers the 24 to 48 hour rest window for that test.
Caffeine, nicotine, alcohol and stimulants
Stimulants are where most people slip, because coffee feels like part of waking up. The published windows vary widely:
| Substance | Ohio State | Academy of Nutrition and Dietetics |
|---|---|---|
| Caffeine | More than 12 to 24 hours | At least 4 hours (with other stimulants) |
| Nicotine | More than 3 hours | Longer than 140 minutes |
| Alcohol | More than 24 hours | Not specified in the summary we reviewed |
| Ephedra, ma huang, pseudoephedrine | More than 12 hours | Not specified in the summary we reviewed |
Sources: Ohio State and the Academy's 2014 recommendations.
Other sites you will encounter set their own numbers, for example 4 or 5 hours for caffeine. Those are shorter than the strictest protocol above, which is why the safest approach for a regular coffee drinker is to skip it entirely that morning and ask your tester what they require. Many pre-workout powders, energy drinks and fat-burner supplements contain caffeine or other stimulants, so read the label. Cold and sinus medicines with pseudoephedrine count too.
If you drink coffee daily, you might get a headache. Plan for it: go to bed earlier, hydrate, and schedule the test first thing so you can have your coffee right after.
Medications and supplements
Do not stop a prescribed medication to "improve" your test. UC Davis tells clients to continue their usual prescriptions, and the same instruction appears in other clinic protocols. What matters is that the tester knows. The Australian Sports Commission's national RMR testing protocol asks that medication that could affect results be kept consistent across testing sessions and documented.
Some drugs do move the number. Per the Academy's review, cardiovascular agents such as beta-blockers can lower RMR by 4 to 12 percent, while thyroxine for hypothyroidism may raise it by as much as 17 percent. That is not a reason to change your dose. It is a reason to take the medication the same way each time you retest, so your results stay comparable. Any question about adjusting a prescription goes to the prescriber who wrote it.
This consistency also matters for the retesting schedule most weight-loss programs use. Our piece on 8 to 12 week retests explains why identical conditions make the change between tests believable.
The day before: sleep, stress and timing
Good sleep and a calm day before help, and UC Davis specifically advises staying rested and hydrated and avoiding intense stress the day before. It also recommends a morning appointment. Their sports medicine team notes that afternoon tests can read about 100 kcal per day higher, probably because a day of activity accumulates (UC Davis).
A simple checklist for the 24 hours before:
- Light day: no hard training, no long, stressful commitments if you can avoid them.
- Normal dinner, no alcohol.
- In bed early. Set an alarm with a buffer so you are not rushing.
- Morning: water only, no coffee, no supplements with stimulants, no nicotine.
- Take regular prescriptions as directed, and write down what you took and when.
- Wear loose, comfortable clothes. You will be lying or sitting still for a while.
Consistency is the thread. If you retest later, repeat the same timing, the same fasting length and the same medication habits.
What happens at the appointment
Knowing the routine takes the edge off. Most sites have you rest first: the Academy's evidence review describes resting supine for about 30 minutes in a quiet, thermoneutral, dimly lit room before measurement begins (review). Many commercial and clinic setups use a shorter rest and a 10 to 20 minute measurement, so your total time will depend on the site.
During the measurement, UC Davis asks you to sit or recline comfortably, stay as still as possible, breathe normally, and avoid talking, laughing or unnecessary movement (instructions). Do not fall into the trap of trying to breathe "correctly." Breathe the way you do on the couch. Do not look at your phone or fidget, and tell the tester if the mask or canopy makes you anxious. A research protocol notes that claustrophobia can produce inaccurate results, so it is better to say so than to tough it out.

Afterward, stand up slowly. You are fasted, and a research protocol flags a small risk of low blood sugar when getting up after a long fast. Bring a snack.
What to do if you ate, trained or had caffeine
It happens. The honest answer is that you have three options, and the right one depends on how big the slip was.

You ate a meal or a substantial snack. Tell the tester and reschedule. A research protocol says that if a participant admits to a recent meal, the test should be rescheduled, and it notes that the respiratory quotient can reveal eating within the last 6 hours. Most clinics will move you without a fuss, and some will do it free.
You had coffee or another stimulant. The same protocol says that if you fasted but had caffeine within 2 to 4 hours of the visit, the test should be rescheduled. If it has been longer than that, the caffeine has likely been metabolized and the test may proceed. Because other sites set stricter windows, defer to your tester's rule. A rescheduled test is cheap compared to a month of eating to a number that was wrong.
You trained hard the day before. Be upfront. Depending on the protocol, strenuous exercise within 24 hours is a reason to move the appointment. Light activity, such as an easy walk, is less of an issue under the ACSM guidance on moderate exercise.
You have to go ahead anyway. If rescheduling is impossible, ask the tester to note the deviation on your report. Then when you retest, you can interpret the difference with the slip in mind, or repeat the test under proper conditions. Do not hide it. A number with a footnote is more useful than a clean-looking number that is wrong.
Pairing an RMR test with a DEXA scan
Many people combine metabolic testing with body composition, because RMR tracks closely with lean mass. Preparation overlaps: fasted, hydrated, no hard exercise. The DEXA scan itself has its own rules, and some sites suggest a consistent hydration state for a more repeatable body-fat reading. Check with the clinic before you book both in a single visit, so the instructions do not conflict.
At Dexascans, we list verified clinics across the country, and many offer RMR and VO2 max as add-ons to a body composition scan. If you are still deciding which measurement answers your question, our overview of energy expenditure measurement methods, accuracy and cost compares the options, and the RMR test cost guide shows what to expect to pay.
Our perspective
We see the same pattern again and again: people spend money on a metabolic test, then undermine it with a coffee or a morning workout, and end up with a number they do not trust. The test is simple. The preparation is where the accuracy comes from.
Our advice is practical. Read your clinic's instructions first, because they are written for their equipment and protocol. Where they are silent, lean toward the stricter published window, since being too careful costs you a little discomfort and being careless costs you the result. Choose a morning slot, and write down exactly what you did so you can repeat it next time. Because a single RMR reading is most valuable as a baseline you compare against, repeatability beats perfection.
We also think you should not have to fight for a straight answer from a clinic. That is why we ask the sites we list to be clear about what scans they offer, what they charge in cash, and whether you need a referral. You should know all of that before you set an alarm for a fasted morning.
— Erik
Book an RMR or DEXA appointment near you
If you are ready to test, Dexascans helps you find a verified clinic by city or ZIP, compare cash prices, check which scan types and add-ons each location offers, and book directly without a physician referral.

Start with our body composition scan page to see how DEXA works and where to book, or head to the Dexascans home page to search your area. Then confirm the clinic's own fasting and exercise instructions when you schedule.
FAQ
How long should I fast before an RMR test?
At least 6 hours is the minimum in the sources we reviewed, and 8 hours is common. The Academy of Nutrition and Dietetics recommends at least 7 hours for healthy adults. Water is allowed, and your site's own instructions take priority.
Can I have coffee before an RMR test?
It is best not to. Published windows run from about 4 hours to 12 to 24 hours, and a research protocol advises rescheduling if you had caffeine within 2 to 4 hours. Ask your tester for their rule, and skip coffee until the test is done.
Should I take my medications before the test?
Yes, unless your prescriber tells you otherwise. Tell the tester what you take, because some drugs, such as beta-blockers and thyroid medication, can shift RMR. Keep your routine the same for any retest so results compare fairly.
Can I exercise the day before an RMR test?
Light activity is generally fine, but avoid strenuous exercise for about 24 hours. Heavy resistance training is the one to watch, since the ACSM paper suggests avoiding it for 14 hours. When in doubt, take a rest day.
What if I messed up my preparation?
Tell the tester before the measurement starts. If you ate or had caffeine recently, rescheduling is the standard advice. If you cannot, ask that the deviation be noted on your report.
Sources
- Resting Metabolic Rate Instructions, UC Davis Sports Medicine
- Resting Metabolic Rate, Ohio State Exercise Science Laboratory
- Metabolic Testing in the Office, ACSM Current Sports Medicine Reports
- EE: Executive Summary of Recommendations, Academy of Nutrition and Dietetics
- RMR Measurement National Testing Protocol, Australian Sports Commission
- Evidence Analysis Library Review of Best Practices for Performing Indirect Calorimetry
- Best practice methods to apply to measurement of resting metabolic rate
- Protocol: Basal Metabolic and Resting Metabolic Rates, PhenX Toolkit
- Fasting requirements for biomarker testing
